Development and Validation of an HPLC Method for Karanjin in Pongamia pinnata linn. Leaves
نویسندگان
چکیده
A rapid, simple and specific reversed-phase HPLC method has been developed for analysis of karanjin in Pongamia pinnata Linn. leaves. HPLC analysis was performed on a C(18) column using an 85:13.5:1.5 (v/v) mixtures of methanol, water and acetic acid as isocratic mobile phase at a flow rate of 1 ml/min. UV detection was at 300 nm. The method was validated for accuracy, precision, linearity, specificity. Validation revealed the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficients (r(2)>0.997) were obtained for calibration plots in the ranges tested. Limit of detection was 4.35 μg and limit of quantification was 16.56 μg. Intra and inter-day RSD of retention times and peak areas was less than 1.24% and recovery was between 95.05 and 101.05%. The established HPLC method is appropriate enabling efficient quantitative analysis of karanjin in Pongamia pinnata leaves.
منابع مشابه
A simple and improved method for isolation of karanjin from Pongamia pinnata Linn. seed oil
Study was undertaken with the objective to develop a simple method for isolation of karanjin from Karanja (Pongamia pinnata Linn.) seed oil. The seed oil was subjected to liquid–liquid extraction with methanol. The extract was further subjected to solvent partitioning followed by crystallization to get karanjin, purity of which was ascertained by HPLC. The purity of isolated karanjin was found ...
متن کاملPhytohemical Screening and Gut Motility Activity of Pongamia Pinnata Bark in Experimental Animal Models
The present study was carried out with the bark of Pongamia pinnata Linn, belonging to the family Fabaceae. It is one of the fast growing, glabrous, deciduous tree and has recently gained importance as a commercial source of furanoflavones like kaempferol, karanjin. Methanolic extract of Pongamia pinnata was subjected to phytochemical screening. Gut motility activity was produced by oral admini...
متن کاملIsoflavonoid glycosides and rotenoids from Pongamia pinnata leaves.
Chromatographic separation of a 70% aqueous methanol extract (AME) of Pongamia pinnata (Linn.) Pierre (Leguminosae) leaves has led to the isolation of two new isoflavonoid diglycosides, 4'-O-methyl-genistein 7-O-beta-D-rutinoside (2) and 2',5'-dimethoxy-genistein 7-O-beta-D-apiofuranosyl-(1"'-->6")-O-beta-D-glucopyranoside (6), and a new rotenoid, 12a-hydroxy-alpha-toxicarol (5), together with ...
متن کاملEffect of leaves on serum lipids in ammonium chloride induced experimental hyperammonemic rats Pongamia pinnata
Original article Hyperammonemia Cholesterol Ammonia Triglycerides Phospholipids Free fatty acids Pongamia pinnata
متن کاملSome Pharmacological Actions of Pongamia Pinnata Linn (karanja)
Ste, bark of pongamia pinnata, a medicinal tree of importance in Ayurveda has been subjected to various pharmacological studies using the aqueous extract in different dose levels. The aqueous extract of bark exhibited significant CNS sedative and antipyretic effects in experimental animals. But there was no effect on cardiovascular system, through it showed antispasmodic effects on smooth muscl...
متن کامل